【摘要】 目的:研究苗药理气活血滴丸对心肌缺血再灌注损伤(MIRI)大鼠心肌的防护机制。方法:将SD大鼠随机分为假手术组、MIRI模型组、理气活血滴丸(175 mg/kg)组和辛伐他汀(40 mg/kg)组。给药组连续灌胃10天,假手术组和MIRI模型组大鼠灌胃等量的生理盐水。末次给药45 min后采用结扎冠状动脉左前降支方法复制MIRI大鼠模型。复灌后监测左心功能;取左心室利用透射电镜观察心肌细胞超微结构;TUNEL-POD法检测心肌细胞凋亡;荧光素酶法检测心肌组织ATP含量;提取心肌细胞线粒体;硫代巴比妥酸法测定心肌细胞线粒体丙二醛(MDA)含量;WST-8法测定线粒体超氧化物歧化酶(SOD)活性情况。结果:与MIRI模型组比较,理气活血滴丸(175 mg/kg)组大鼠左心功能和左心室肌细胞超微结构明显改善;线粒体损伤减轻;细胞凋亡发生率减少;心肌组织ATP含量升高;心肌细胞线粒体MDA含量降低;SOD活性升高。结论:理气活血滴丸对MIRI模型大鼠的防护机制与其改善左心室肌细胞超微结构、抑制细胞凋亡和减轻线粒体氧化损伤有关。更多还原

【Abstract】 Objective: To study the protective mechanism of Liqihuoxue dripping pill against myocardial ischemia reperfusion injury(MIRI) in rats. Methods: Sprauge-Dawley rats were randomized into four groups,including the sham group,MIRI model group,Liqihuoxue dripping pill(175 mg/kg) group and simvatatin(40 mg/kg) group. Rats in each group were intragastrically given drugs for 10 days,once a day.Rats in the sham group and model group were given the same amount of saline. MIRI rat model was established by ligating the left anterior descending coronary artery 45 minutes after gavage. Left ventricular function was observed after ischemia-reperfusion,and left ventricles were collected. The ultrastructure of the cardial myocytes was observed. Apoptosis of myocardial cells was detected by TUNEL. ATP level of myocardial cells was determined by luciferase method. After the mitochodria dissociated,MDA content in the mitochondria was measured by thiobabituric acid assay,and the activity of superoxide dismutase(SOD) was measured by WST-8. Results: The left ventricular function and left ventricular myocytes ultrastructure were significantly improved in Liqihuoxue dripping pill group compared with those in the model group. Moreover,mitochonodrial injury was obviously ameliorated,the incidence of apoptosis was significantly reduced and the content of ATP in myocardium tissues was obviously high,compared with those in the model group,respectively. The content of MDA in myocardial cells mitochondria was decreased and the activity of SOD was increased. Conclusion: The protective mechanism of Liqihuoxue dripping pill on MIRI model rats is related to improving the ultrastructure of left ventricular myocytes,inhibiting apoptosis and mitigating mitochondrial oxidative damage.更多还原