【摘要】 目的:上皮细胞迁移是胃肠黏膜损伤修复的重要环节,观察白术、黄芪糖复合物对小肠上皮IEC-6细胞迁移多胺信号通路钾通道相关指标的影响,为探讨益气健脾中药白术、黄芪胃肠黏膜损伤修复机制提供参考。方法:划痕法复制细胞迁移模型;非损伤微测法检测细胞K~+外流;Western Blot法检测Kv1.1蛋白表达;HE染色观察细胞形态;透射电镜观察细胞超微结构。结果:白术、黄芪糖复合物具有以下作用:50μg/ml均可促进细胞迁移;100μg/ml均能增加细胞K+外流;白术(50μg/ml)、黄芪(100μg/ml)糖复合物能提高钾通道蛋白Kv1.1蛋白表达;200μg/ml剂量均能逆转钾通道抑制剂4-氨基比啶(4-AP)所致的细胞迁移抑制;100、200μg/ml剂量均能改善4-AP所致的Kv1.1蛋白表达抑制和细胞空泡化。结论:白术、黄芪促进胃肠黏膜损伤修复的作用与其影响小肠上皮细胞迁移多胺信号通路钾通道相关指标有关。更多还原

【Abstract】 Objective: To investigate effects of glycoconjugates from Atractylodes macrocephala and Astragalus membranaceus on polyamine mediated potassium signaling pathway during rats’ intestinal epithelial cell(IEC-6) migration,and to explore the mechanism of Atractylodes macrocephala and Astragalus membranaceus on promoting gastrointestinal mucosal repair after injury. Methods: Cell migration model was established by scratch damage. Non-invasive Micro-test Technique was used to detect the potassium efflux in cells. The protein expression of Kv1.1 was assessed by Western Blot. Cell morphology was observed by HE staining. Transmission electron microscope was used to observe the cell ultrastructure. Results: Glycoconjugates from Atractylodes macrocephala and Astragalus membranaceus promoted cell migration,(50μg/ml,P < 0. 01,compared with the control group) and increased the potassium efflux in cells(100μg/ml P < 0. 01,compared with the control group). Glycoconjugates from Atractylodes macrocephala(50μg/ml) and Astragalus membranaceus(100μg/ml) also enhanced the expression of protein Kv1. 1. Besides,two Glycoconjugates reversed the inhibition of cell migration(200μg/ml,P < 0. 01,compared with the 4-AP group) as well as improved the reduction of expression of protein Kv1. 1(100 and 200μg/ml) and vacuole formation(100 and 200μg/ml) induced by potassium channel inhibitor 4-AP. Conclusion: Atractylodes macrocephala and Astragalus membranaceus that promoting gastrointestinal mucosal repair after injury is related to its role of regulating relevant targets in polyamine mediated potassium signaling pathway during intestinal epithelial cell migration.更多还原