【摘要】 目的:建立同时测定臭冷杉挥发油中柠檬烯和醋酸龙脑酯含量的方法。方法:采用气相色谱-质谱联用(GC-MS)技术,选择离子模式(SIM)检测,色谱柱为HP-5MS石英毛细管柱(30 m×0.25 mm×0.25μm),初始温度40℃,4℃·min-1升至260℃。载气为氦气。选择m/z为68和95的特征离子分别对柠檬烯和醋酸龙脑酯进行定量。结果:柠檬烯和醋酸龙脑酯在400~4 000μg·m L-1和800~8 000μg·m L-1浓度范围内与峰面积呈良好的线性关系,其相关系数为0.999 8和0.999 6;平均加样回收率为98.5%和98.7%;RSD值为1.3%和1.5%(n=6)。结论:该方法简便、快速,结果准确,可用于臭冷杉挥发油的质量控制。更多还原

【Abstract】 Objective: To develop a new method for the determination of limonene and bornyl acetate content in the essential oil from Abies nephrolepis. Method: The limonene and bornyl acetate were analyzed by gas chromatography-mass spectrometry( GC-MS)/SIM on the HP-5 MS quartz capillary chromatographic column( 30 m × 0. 25 mm × 0. 25 μm) and quantified by selective ion monitoring( SIM). Initial temperature was set at 40 ℃. It was programmed rising by 4 ℃ ·min-1 until 260 ℃. The selected ions were m/z 68 and 95. Results: A good linear relationship was achieved for limonene and bornyl acetate over the range of 400-4 000 μg ·m L-1( r =0.999 8) and 800-8 000 μg ·m L-1( r =0.999 6). The average recovery was 98. 5%( RSD = 1. 3 %,n = 6) and 98. 57%( RSD = 1. 5 %,n = 6). Conclusion: The method is simple,rapid and accurate,and suitable for the quality control of the essential oil from Abies nephrolepis.更多还原