【摘要】 目的:探讨鲜肤膏及其拆方对2,4-二硝基氯苯（DNCB）诱导小鼠慢性湿疹的量效关系,确定半数有效剂量(ED₅₀)及方剂配伍增效。方法:DNCB诱导C57小鼠慢性湿疹,给予梯度剂量鲜肤膏(11.71¹1 662.50 mg·kg^-1·d^-1,k=0.316)、地乌(0.53⁵30.12 mg·kg^-1·d^-1,k=0.316)、鲜肤膏去地乌(11.18¹1 132.40 mg·kg^-1·d^-1,k=0.316)治疗,HE染色观察病理特点,ELISA检测血清白细胞介素2（IL-2）、干扰素-γ（IFN-γ）、白细胞介素4（IL-4）、白细胞介素13（IL-13）含量;Prism 5.0非线型可变斜率回归计算ED₅₀,比较鲜肤膏、地乌、鲜肤膏去地乌组的药效,观察地乌的配伍增效作用。结果:鲜肤膏、地乌、鲜肤膏去地乌组药效具有剂量依赖特征,量效曲线呈"S"型。鲜肤膏组疗效最优,显著降低表皮厚度(ED₅₀=377.90mg·kg^-1·d^-1),减少真皮淋巴细胞浸润数(ED₅₀=153.20 mg·kg^-1·d^-1),增加血清IL-2(ED₅₀=608.90 mg·kg^-1·d^-1)、IFN-γ(ED₅₀=205.50 mg·kg^-1·d^-1)含量,降低血清IL-4(ED₅₀=198.70 mg·kg^-1·d^-1)、IL-13(ED₅₀=117.60mg·kg^-1·d^-1)含量。与鲜肤膏比较,地乌组与鲜肤膏去地乌组的量效曲线分别右移。结论:鲜肤膏及其拆方均能有效治疗湿疹;地乌有配伍增效作用,鲜肤膏组的疗效最优。更多还原

【Abstract】 Objective:To investigate the dose-effect relationship of Xianfu ointment and its decomposed recipes the1-chloro-2,4-dini-trochlorobenzene（DNCB）induced chronic eczema in mice,and confirm the median effective dose(ED₅₀)of each formula and the synergetic effect by compatibility.Methods:DNCB was used to induce chronic eczema in C57 mice.The mice were treated with gradient dosages of the Xianfu ointment(11.71-11 662.50 mg·kg^-1·d^-1,k=0.316),Anemone flaccid(0.53-530.12 mg·kg^-1·d^-1,k=0.316),Xianfu ointment without Anemone flaccid(11.18-11 132.40 mg·kg^-1·d^-1,k=0.316),respectively.The pathological features were observed after hematoxylin-eosin staining.The volume ratio of epidermides and the number of lymphocyte infiltrated in dermis were analyzed with morphometry.The serum levels of IL-2,IFN-γ,IL-4,and IL-13 were detected by ELISA assay.The ED₅₀was calculated by non-linear regression with various slope using Prism-5.0software.Results:The effects of Xianfu ointment and its decomposed recipes on chronic eczema showed a dose-dependent tendency.The dose-response curves showed"S"shape.The efficacy of Xianfu ointment on chronic eczema was the most significant among the three formulas,which was demonstrated by decreased epidemical thicknes(ED₅₀=377.90mg·kg^-1·d^-1),reduced infiltrated lymphocyte number(ED₅₀=153.20 mg·kg^-1·d^-1),increased serum IL-2(ED₅₀=608.90 mg·kg^-1·d^-1)and IFN-γ(ED₅₀=205.50 mg·kg^-1·d^-1)levels,and decreased serum IL-4(ED₅₀=198.70mg·kg^-1·d^-1)and IL-13 levels(ED₅₀=117.60 mg·kg^-1·d^-1).And the dose-effect curves of Anemone flaccid and Xianfu ointment without Anemone flaccid groups were both right shift when compared with that of Xianfu ointment.Conclusion:Xianfu ointment and its decomposed recipes can effectively treat chronic eczema.Anemone flaccid has obvious compatibility synergy in the whole formula.The effects of Xianfu ointment is most significant.更多还原