【摘要】 目的:建立氢化物发生-原子荧光光谱法（HG-AFS）和高效液相色谱-氢化物发生-原子荧光光谱法（HPLC-HG-AFS）分别测定急性早幼粒细胞白血病患者红细胞和血浆中总砷及血浆中无机砷[As（Ⅲ）和As（V）]和甲基化代谢产物（MMA和DMA）的浓度。方法:将患者红细胞和血浆经HNO₃-H₂O₂的混合物消化处理后,利用HG-AFS法检测血浆和红细胞中总砷的浓度。将患者血浆经高氯酸沉淀蛋白,取上清液进行HPLC-HG-AFS分析,色谱柱为Hamilton PRP-X100阴离子交换色谱柱（250 mm×4.1 mm,10μm）;以13 mmol·L^-1醋酸钠-3 mmol·L^-1磷酸二氢钠-4 mmol·L^-1硝酸钾-0.2 mmol·L^-1乙二胺四乙酸二钠的混合溶液为流动相。结果:总砷的线性范围为0.2²0 ng·ml^-1（r=0.999 7）,4种砷形态的线性范围为2.0⁵0ng·ml^-1（r>0.9950）。红细胞中总砷加样回收率为89.4%¹06.3%;血浆中加样回收率为87.6%¹00.2%;砷形态加样回收率为81.2%¹08.6%。精密度良好。该方法成功应用于5例急性早幼粒细胞白血病患者体内总砷以及砷形态的分析。结论:所建立的方法简便、快速、准确。可应用于急性早幼粒细胞白血病患者血中总砷及砷形态的检测。更多还原

【Abstract】 Objective:To quantify the plasma concentrations of inorganic arsenic（As（III）and As（V））and methylated metabolites（MMA and DMA）,and to detect the total amount of arsenic in blood cells and plasma by high performance liquid chromatography-hydridegeneration-atomic fluorescence spectrometry（HPLC-HG-AFS）and HG-AFS methods to clarify the arsenic species in acute promyelocytic leukemia（APL）patients.Methods:The blood cells and plasma were digested by the mixture of HNO₃-H₂O₂and analyzed by HG-AFS.For the arsenic species,the plasma samples were prepared with perchloric acid to precipitate protein.The supernatant was separated on an anion-exchange column in 6 min with isocratic elution using 13 mmol·L^-1CH₃COONa,3 mmol·L^-1Na H₂PO₄,4 mmol·L^-1KNO₃and 0.2 mmol·L^-1EDTA-2Na.Results:The methods provided linear range of 0.2-20 ng·ml^-1for total arsenic and 2.0-50 ng·ml^-1for four arsenic species（r>0.9950）.The spiked recoveries ranged from 81.2%to 108.6%,and the coefficients of variation for intra-and inter-batch precision were less than 9.3%and 12.5%,respectively.The developed methods were applied successfully in the assay of total arsenic and arsenic species in 5 APL patients.Conclusion:The method is simple,fast and accurate,which can be applied in the assay of arsenic compounds in plasma and blood cells in APL patients.更多还原