【摘要】 以黄瓜(Cucumis sativus L.)嫩果皮绿色自交系1613(P1)和嫩果皮白色自交系JD7(P2)为试验材料构建重组自交系(RIL)群体(142个株系),对各株系嫩果皮色进行表型鉴定,利用重测序技术对各自交系进行基因分型,结合表型和基因型数据进行QTL定位研究。结果表明F1黄瓜嫩果皮颜色表现为绿色,并将控制嫩果皮颜色的基因定位到黄瓜第3号染色体上,在3号染色体35511129~39711114区段内定位到成簇分布的3个位点,它们在连锁图谱上的位置分别为1. 01 c M、3. 31 c M和6. 01 c M处,与两翼标记的连锁距离分别为1. 01 c M/0. 09 c M,0. 21 c M/0. 29 c M,0. 11 c M/0. 19 c M。通过生物信息学分析预测出16个候选基因,其中Csa3G904080、Csa3G904100、Csa3G903500和Csa3G902950极有可能是调控黄瓜果实颜色的关键基因。更多还原

【Abstract】 By taking use of 142 recombinant inbred lines(RILs) derived from a cross of green immature fruit inbred line(female parent,1613,P1) and white immature fruit inbred line(male parent,JD7,P2),we performed the quantitative mapping of traits that control the immature fruit skin color. F1 plants showed green peel in immature fruit. By sequencing technology,three QTLs controlling the immature fruit color were allocated to the physical interval of 35511129-39711114 on chromosome 3. These loci were located at 1. 01 cM,3. 31 cM and 6. 01 cM,with the flanking genetic distance of 1. 01 cM/0. 09 cM,0. 21 cM/0. 29 cM and 0. 11 cM/0. 19 cM,respectively. By gene annotation,16 candidate genes were found in the target region,including the genes Csa3 G904080,Csa3 G904100,Csa3 G903500 and Csa3 G902950,which are assumed to be the key genes that regulate the color of cucumber fruit.更多还原