【摘要】 本文采用水培方法,研究不同铵硝配比营养液对枳橙（Citrus sinensis×Poncirus trifoliate）幼苗体内谷氨酰胺合成酶（GS）活性变化及相关基因的表达量变化的影响。结果表明:不同处理的枳橙幼苗叶片GS酶活性均随着培养时间的延长而有一定增加,其中NO₃^-、NO₃^-:NH4+=7:3、NO₃^-:NH₄⁺=1:1和NO₃^-:NH₄⁺=3:7处理的GS酶活性大小无显著差异, NH₄+处理最小。各处理幼苗根系的GS活性显著高于叶片,其中NO₃^-:NH₄⁺=7:3和NO₃^-:NH₄⁺=1:1最高。3个GS相关基因CitGln 1.3、CitGln HZ、CitGln 2在幼苗各部位中均有表达。其中根系中CisGln HZ的表达量最高,叶片中CisGln HZ和CitGln 2表达量显著高于CisGln 1.3。由此推测CisGln HZ是存在于幼苗根系和叶片中GS酶的主要组成基因,并且受到氮素形态及配比的调控,对该基因进行系统进化树分析结果表明该基因与GS1家族功能最为相近。更多还原

【Abstract】 Hydroponic experiments were carried out to study the influences of the different ratio of NO₃^-:NH₄⁺on the citrange（Citrus sinensis×Poncirus trifoliate） seedling glutamine synthetase（GS） activity changes and relative genes expression. The results showed that the GS activities of citrange leaves in all treatments were increasing as the culture time continuing. The GS activities in treatments of NO₃^-, NO₃^-:NH₄⁺=7:3, NO₃^-: NH₄⁺=1:1 and NO₃^-:NH₄⁺=3:7 had no significant difference in each other, and that in treatment of NH₄⁺ was lowest. Under all treatments the GS activities in roots were significantly higher than those in leaves, and the GS activities of NO₃^-:NH₄⁺=7:3 and NO₃^-:NH₄⁺=1:1 were highest. Three GS relative genes（CitGln 1.3, CitGln HZ, CitGln 2） were all detected in citrange seedling. The expression of CitGln HZ was highest in roots, while the expression of CisGln HZ and CitGln 2 in leaves were higher than CitGln 1.3. And it was induced that CisGln HZ was the corn genes of GS in the roots and leaves. Phylogenetic trees analysed indicated that CitGln HZfunction was similar to GS1 family.更多还原