【摘要】 以金佛山兰子房、柱头等为外植体,通过丛芽诱导、继代增殖和生根建立离体繁殖体系。结果表明,丛芽诱导最佳外植体为子房,最适培养基为MS+2.0 mg·L^-1 ZT+0.2 mg·L^-1 NAA;最佳丛芽继代培养基为MS+1.5mg·L^-1 ZT+0.15 mg·L^-1 NAA, 1～4代平均繁殖系数为4.76倍,丛芽长势良好;最佳生根培养基为1/2MS+0.5 mg·L^-1NAA+0.05mg·L^-16-BA,生根率达100%,根长为3.95cm。试管苗移栽到金佛山自然生境,60d后的移栽率达60%,且生长良好。更多还原

【Abstract】 In vitro propagation system was established by using ovary, stigma, etc. of Tangtsinia nanchuanica as explants. The results showed that the optimal explants were ovary, and the optimal medium was MS+2.0 mg·L^-1 ZT+0.2 mg·L^-1 NAA. The optimal subculture medium was MS+1.5 mg·L^-1 ZT+0.15 mg·L^-1 NAA. The average reproduction coefficient of 1 to 4 generations was 4.76 times, and the bud grew well. The optimal rooting medium was 1/2 MS+0.5 mg·L^-1 NAA+0.05 mg·L^-1 6-BA with rooting rate of 100% and root length of 3.95 cm. Plantlets in vitro were then transplanted to the natural habitat of Jinfo Mountain, the survival rate was 60% and plants grew well after 60 days.更多还原