【摘要】 本文以陆地棉(Gossypium hirsutum)‘石远321’为实验材料,采用实时荧光定量PCR技术,研究了经甜菜夜蛾(Spodoptera exigua)取食诱导和茉莉酸甲酯(MeJA)处理的茉莉酸(JA)合成途径中关键基因及萜类合酶基因的时间表达模式。甜菜夜蛾取食陆地棉后,JA合成途径脂氧合酶基因(GhLOX1和GhLOX2)、丙二烯氧化物合酶基因(GhAOS)、丙二烯氧化物环化酶基因(GhAOC)随处理时间变化均有不同程度的上调表达,其中GhLOX2表达量上调最明显,处理后12和72h表达量分别上升64.4和118.7倍;5个萜类合酶基因GhTPS1、GhTPS2、GhTPS3、GhTPS4、GhTPS5随处理时间变化表达模式明显不同,GhTPS4和GhTPS5表达量明显升高。外源MeJA处理后,GhLOX2表达量急剧上升,变化最大;5个萜类合酶基因均受MeJA诱导表达,但表达量在处理后不同时间有明显差异,GhTPS4处理后各时间点的表达量均高于对照。这些结果表明JA合成途径的GhLOX2和萜类合酶基因GhTPS4是响应甜菜夜蛾取食诱导和MeJA处理最为重要的基因。更多还原

【Abstract】 In this study, ‘Shiyuan 321’, a variety of upland cotton(Gossypium hirsutum), was used to investigate the impact of Spodoptera exigua feeding and methyl jasmonate(Me JA) treatment on the expression profiles of genes involved in the biosynthesis of jasmonic acid(JA) and terpene. Feeding damage inflicted by S. exigua caused the increased expression levels of GhLOX1, GhLOX2, GhAOS and GhAOC. Specially, the expression levels of GhLOX2 were 64.4-fold and 118.7-fold higher than the control cotton by 12 and 24 hours after feeding. In addition, five terpene synthase(TPS) genes, named as GhTPS1, GhTPS2, GhTPS3, GhTPS4 and GhTPS5, exhibited distinct expression profiles after feeding, of which GhTPS4 and GhTPS5 were up-regulated by S. exigua feeding. Me JA induced a significant increase in GhLOX2 transcript levels, and also promoted terpene synthase expression at different stages after treatment. Treating in vitro with Me JA induced the significantly different expression of GhTPS4 during various later stages. In conclusion, our data demonstrate that GhLOX2 and GhTPS4 are the most important genes induced by S. exigua feeding and MeJA treatment in G. hirsutum.更多还原