【摘要】 目的通过对六安地区小肠结肠炎耶尔森菌的病原学研究及流行病学分析,为做好该菌的防控提供科学依据。方法采集腹泻患者、动物粪便、冷冻冷藏食品、苍蝇等样本,进行分离培养、生化鉴定以及分子生物学检测。结果小肠结肠炎耶尔森菌的总检出率为7. 35%,腹泻患者粪便检出率最低,仅为0. 81%,动物粪便检出率由高到低依次为猪、鸭、鹅、狗、鸡,但只有猪粪标本和鸭粪标本检出致病菌株。冷冻冷藏食品检出率为16. 05%。致病菌株O∶3型毒力基因分布为ail+ystA+ystB-yadA+virF+RFBC+。结论该菌在本地区腹泻患者和动物粪便、食品、苍蝇中普遍存在。主要致病型为O∶3型,动物宿主主要是家猪。更多还原

【Abstract】 Objective Through the pathogenic research and epidemiological analysis of Yersinia enterocolitica in Lu’ an,to provide scientific basis for the prevention and control of the bacteria. Methods Feces were collected from diarrhea patients and animals. Freeze frozen food,flies and other samples were collected for isolation,culture,biochemical identification and molecular biology testing. Results The total detection rate of Yersinia enterocolitica was 7. 35%,and the detection rate of feces in diarrhea patients was the lowest,only 0. 81%. The detection rate of animal feces from high to low was pig,duck,goose,dog,chicken. However,only pathogenic strains were detected from pig manure specimens and duck feces specimens. The detection rate of frozen and chilled food was 16. 05%. The virulence gene distribution of pathogenic strain( O∶3) was ail + ystA + ystB-yad A + virF + RFBC +. Conclusion Yersinia enterocolitis is pervasive in human and animal feces,food and flies. O ∶3 serotype is the main type,and pigs are the main hosts.更多还原