【摘要】 为明确引起马铃薯黄萎病的大丽轮枝菌Verticillium dahliae的遗传变异和致病力的差异,对从内蒙古及其周边地区马铃薯发病株上分离得到的29株大丽轮枝菌的营养亲和群、生理小种和交配型进行了测定,利用伤根接种法进行致病力分化研究。结果表明,供试的29株马铃薯大丽轮枝菌菌株被划分为VCG2B、VCG4B和VCG4A三个营养亲和群,其菌株数分别为11、2和16株;供试的29株菌株均鉴定为单一的2号生理小种和MAT1-2-1交配型。供试的29株大丽轮枝菌菌株间致病力存在一定的差异,其中NCP-1菌株的致病力最强,病情指数为83.33;而NWS-5菌株的致病力最弱,病情指数仅为10.85;不同营养亲和群菌株的平均致病力也存在显著差异,VCG4B型菌株的平均致病力最高,平均病情指数为67.18;其次为VCB2B型菌株,平均病情指数为42.50,而VCG4A型菌株致病力最弱,平均病情指数仅为20.54。更多还原

【Abstract】 In order to clarify the genetic variation and pathogenicity differentiation in the pathogenic bacteria of potato Verticillium wilt, 29 strains were isolated from diseased samples which were collected from different potato fields in Inner Mongolia and their surrounding areas, and then the VCG types,physiological races, mating types and pathogenicity differentiation were measured and the pathogenicity of all tested strains was studied by using root-drenching method. The results showed that 29 strains could be divided into three VCGs, namely VCG2 B(11 strains), VCG4 B(two strains), and VCG4 A(16 strains), which were identified as Race 2 and MAT1-2-1. There were significant differences among the tested strains, and the pathogenicity of NCP-1 strain was the highest, with a disease index of 83.33; the pathogenicity of NWS-5 strain was the lowest, with a disease index of 10.85. There was significant difference between the pathogenicity of the three VCGs, and the pathogenicity of VCG4 B was the highest,with an average disease index of 67.18, followed by VCG2 B group, with an average disease index of42.50, and the lowest pathogenicity was observed in VCG4 A, with an average disease index of 20.54.更多还原