【摘要】 目的探讨滤泡辅助性T淋巴细胞(follicular helper T lymphocyte,Tfh)细胞及其转录因子无刚毛鳞甲复合体样蛋白2(achaete-scute homologue 2,Ascl2)在慢性HBV感染中的表达和意义。方法选择CHB患者40例,健康对照者30名。采用流式细胞术检测CD4~+CXCR5~+Tfh细胞及CD4~+CXCR5~+PD-1~+Tfh细胞频数;实时定量PCR法检测Tfh细胞中Ascl2表达水平;ELISA检测血清中各细胞因子水平。结果与健康对照者相比:CHB患者的CD4~+CXCR5~+Tfh及CD4~+CXCR5~+PD-1~+Tfh细胞频数升高,差异有统计学意义(t=4.786,P<0.05);CHB患者中Ascl2mRNA表达升高,差异有统计学意义(t=4.655,P<0.05);CHB患者的IL-21、IL-17均升高,差异均有统计学意义(t=4.896和t=3.764,P<0.05)。结论 Ascl2可能与HBV感染者Tfh细胞的分化发育有关。更多还原

【Abstract】 Objective To investigate the significance of achaete-scute homologue 2(Ascl2)expression in T follicular helper(Tfh)cells during chronic hepatitis B virus infection.Methods Frequencies of CD4~+CXCR5~+Tfh cells and CD4~+CXCR5~+PD-1~+Tfh cells in peripheral blood mononuclear cells(PBMC)were analyzed by flow cytometry,respectively.The expression of Ascl2 in Tfh cells was determined by quantitative real-time polymerase chain reaction.The levels of serum interleukin-21(IL-21)and interleukin-17(IL-17)were measured using enzyme-linked immunosorbent assay.Results The frequencies of CD4~+CXCR5~+and CD4~+CXCR5~+PD-1~+Tfh cells were significantly higher in PBMC from chronic hepatitis B(CHB)patients than that from healthy controls(t=4.786,P<0.05).The expression of Ascl2 was significantly higher in Tfh cells of CHB patients(t=4.655,P<0.05).Furthermore,the levels of serum IL-21 and IL-17 were significantly higher in CHB patients(t=4.896 and t=3.764,P<0.05).Conclusion It was implied that transcription factor Ascl2 plays an important role in the differentiation and development of Tfh cells in CHB patients.更多还原