【摘要】 为了建立HPLC同时测定介孔SiO₂/脂质复合递药系统（LMSNs）中紫杉醇（TAX）与姜黄素（CUR）含量的方法。方法:Eclipse XDB-C18柱（150 mm×4.6 mm,5μm）,乙腈与0.1%甲酸水溶液以不同比例梯度洗脱,流速1 mL/min,检测波长227 nm,柱温25℃,进样体积5μL。结果:TAX、CUR分别均在1.5³0μg/mL、6³00μg/mL范围内线性良好（TAX低、高浓度范围R²各为0.999 7、1.000 0,CUR低、高浓度范围R²各为0.999 9、0.999 9）;TAX低、中、高浓度回收率各为（95.60±1.53）%、（98.83±1.02）%、（101.49±0.70）%,CUR为（101.44±0.04）%、（103.10±1.04）%、（103.66±0.47）%（n=3）。表明本方法准确度高、重复性好,可用于同时测定TAX-CUR介孔SiO₂/脂质复合递药系统中两药含量。更多还原

【Abstract】 To establish a method for the simultaneous determination of Taxol and Curcumin in mesoporous SiO₂/lipid complex drug delivery systems by HPLC. Methods: The determination was performed on Eclipse XDB-C18（ 150 mm × 4. 6 mm,5 μm） column with mobile phase composed of acetonitrile and 0. 1% formic acid aqueous solution at the flow race of 1. 0 mL·min-1. The detection wavelength was set at 227 nm,column temperature was 25 ℃ and injection volume was 5 μL. Results: The linear ranges of TAX and CUR were 1. 5 30 μg/mL and 6 300 μg/mL（ TAX R² =0. 999 7,1. 000 0. CUR R²= 0. 999 9,0. 999 9）,and the average recoveries of TAX and CUR in three concentration levels were（ 95. 60 ± 1. 53） %,（ 98. 83 ± 1. 02） %,（ 101. 49 ± 0. 70） %,CUR（ 101. 44 ± 0. 04） %,（ 103. 10 ± 1. 04） %,（ 103. 66 ± 0. 47） %（ n = 3）. Conclusion: The method is accurate,rapid and reproducible for the concentration determination of Taxol and Curcumin in mesoporous SiO₂/lipid complex drug delivery systems at the same time.更多还原