【摘要】 细胞色素P450是超基因家族,属于生物体内重要三大解毒酶系之一,参与内源物和外源物的合成与氧化代谢。本文通过棉铃虫溴氰菊酯短期胁迫转录组数据分析,克隆获得一条P450基因CYP9G5(Gen Bank:KM016757.1),编码493个氨基酸,分子量约为57.930 k Da,理论等电点p I 7.16。通过实时荧光定量技术,分析CYP9G5基因在棉铃虫不同组织和溴氰菊酯处理后表达量的变化,发现CYP9G5在头部表达量最高,经过溴氰菊酯处理后CYP9G5表达量也显著升高。推测出CYP9G5极有可能参与棉铃虫对溴氰菊酯的代谢,可能对棉铃虫的耐药性有一定的作用。本研究为进一步探索棉铃虫的抗药性和新农药的开发奠定了基础。更多还原

【Abstract】 Cytochrome P450 is a supergene family, which belongs to one of the three important detoxification enzymes in vivo and participates in the synthesis and oxidation metabolism of endogenous and exogenous substances. In this paper, a P450 gene CYP9 G5(Gen Bank: KM016757.1) was cloned through data analysis of the transcription group of H. armigera, encoding 493 amino acids with a molecular weight of about 57.930 k Da, and theoretical isoelectric point p I 7.16. Using the technology of real-time quantitative analysis the CYP9 G5 genes in different groups of the Helicoverpa armigera and deltamethrin treatment after expressing quantity change, found CYP9 G5 highest expression quantity of the head, after treated with deltamethrin CYP9 G5 express volume also increased significantly. It is speculated that CYP9 G5 is very likely to be involved in the metabolism of H. armigera to cypermethrin, and may play a role in the resistance of H. armigera. This study lays a foundation for further exploring the resistance of H. armigera and the development of new pesticides.更多还原