【摘要】 目的探讨染料木黄酮（GEN）对小鼠卵母细胞成熟的影响。方法以小鼠为实验动物,随机分为4组,分别给予不同剂量（0、0.5、5.0、50.0 mg/kg BW）的GEN处理7 d,观察GEN对小鼠促排卵和生殖激素水平[雌二醇（E₂）、黄体生成素（LH）和卵泡刺激素（FSH）]的影响;同时,进行体外培养试验,用不同浓度的GEN处理体外培养卵丘-卵母细胞复合物（COCs）和裸卵（DOs）,观察GEN是否抑制卵母细胞的减数分裂成熟过程。结果 GEN处理可降低小鼠促排卵数,但对机体生殖激素（包括E₂、FSH和LH）水平无影响;体外培养过程中,GEN可抑制COCs中卵细胞的减数分裂成熟过程,但对DOs的成熟过程无明显影响。结论 GEN可干扰小鼠卵母细胞的成熟过程,其作用机制可能是发生在卵巢中。更多还原

【Abstract】 Objective To investigate the toxicity of genistein（ GEN） on oocyte maturation in mice. Methods In vivo test,mice were randomly divided into 4 groups and administrated with genistein（ 0,0. 5,5. 0 and 50. 0 mg/kg BW） for7 days. After superovulation,the ovulated oocytes picked up from oviduct ampulla were counted,and the estradiol（ E₂）,follicle stimulating hormone（ FSH） and luteinizing hormone（ LH） in serum were detected. During in vitro test,cumulusoocyte complex（ COCs） and denuded oocytes（ DOs） were collected from super-ovulated mice and cultured by different concentrations of GEN to evaluate the influence of GEN on oocyte meiotic maturation. Results The number of superovulated oocytes in animals was significantly decreased by GEN treatment,while sex hormone remained unchanged.GEN produced an evident impact on the meiotic process of in vitro cultured COCs but DOs. Conclusion Our result confirmed the influence of GEN on oocyte maturation of mice,with its potential mechanism in ovary.更多还原