We studied the CRISPR genotypes data and regional distribution of Y.pestis strains in Sichuan Province,and provided a scientific basis for prevention and treatment of plague.For determining the CRSIPR genotype,we cultivated and extracted nucleic acid from 132 Y.pestis strains which isolated from plague foci of Sichuan Province.Using PCR amplification,sequencing and analysis,all strains were genotyped with YPa,YPb and YPc primers of CRISPR.Results showed that we found 17 spacers,including 9 of YPa,5 of YPb a...
【基金】
四川省卫计委课题(No.16PJ397)资助~~
【更新日期】
2018-11-14
【分类号】
R378
【正文快照】
(1.Sichuan Center for Disease Control and Prevention,Chengdu 610041,China;2.China Center for Disease Control and Prevention,Beijing102206,China;3.Ganzi Tibeian Autonomous Prefecture Center for Disease Control and Prevention,Kangding626000,China;4.Shiqu Ce