【摘要】 本研究旨在明确IAA和ABA在甜菜发育过程中的作用机制。用生物信息学方法鉴定出IAA和ABA生物合成酶基因,通过q RT-PCR检测基因的表达并结合超高效液相色谱质谱(UPLC/MS/MS)定量分析IAA和ABA的浓度变化。结果表明BvIAA6、BvIAA26和BvIAA33基因在甜菜茎和花蕾中协同调节IAA激素含量的变化,BvABA2、BvABA3、BvNCED1和BvNCED5基因调控ABA的含量变化;研究发现ABA的含量高于IAA,但是IAA在促进植物生长方面的作用强于ABA;IAA和ABA生物合成酶基因具有组织特异性表达的特征。研究阐明了内源性IAA和ABA在甜菜生殖生长过程中的分子机制,为作物的分子育种提供了应用性参考。更多还原

【Abstract】 This study aims at clarifying the molecular mechanisms of IAA and ABA in the development of sugar beet. The IAA and ABA biosynthesis genes were identified by bioinformatics. The gene expression was detected by q RT-PCR, and the concentrations of IAA and ABA were analyzed quantitatively by UPLC/MS/MS.Results showed that BvIAA6, BvIAA26 and BvIAA33 coordinately regulated IAA concentration, while ABAconcentration was controlled by BvABA2, BvABA3, BvNCED1 and BvNCED5. ABA concentration was higherthan that of IAA, but IAA was more powerful to promote plant growth than ABA. IAA and ABA biosynthesis genes had tissue specific expression. These findings elaborate the molecular mechanism of IAA and ABA in reproductive growth of sugar beet and provide references for crop breeding.更多还原