Streptomyces Z331-A is a biocontrol strain with good activity. In order to improve the antibacterial effect of Streptomyces Z331-A fermentation broth, the fermentation conditions were optimized. On the basis of single factor experiments, the three key factors(including fermentation time,inoculum, shaker speed) affecting the fermentation of strain Z331-A were selected by Plackett-Burman design. Then the optimum fermentation conditions were determined by response surface experiments. The results showed that t...