为了比较分析野生酵母菌产β-葡萄糖苷酶的情况,筛选高产β-葡萄糖苷酶的酵母菌株,该研究采用七叶灵显色法和4-硝基苯基-β-D-吡喃葡萄糖苷(p-NPG)显色法对从宁夏贺兰山东麓分离的941株野生酵母的产β-葡萄糖苷酶能力进行筛选,通过WL培养基上的酵母菌落形态和26S r DNA D1/D2区的序列分析对所有酵母菌株进行分类鉴定,并且对酵母产β-葡萄糖苷酶能力进行差异性分析。结果表明,941株酵母被鉴定为14个种,且筛选出了4株酵母菌高产β-葡萄糖苷酶:菌株SLY-4(98.51 U/L)、F2-24(76.93 U/L)、F2-16(62.72 U/L)和HX-13(47.95 U/L)。产酶能力差异性分析表明β-葡萄糖苷酶广泛分布于14种酵母,但是产酶能力表现出明显的种间和种内差异性。
【英文摘要】
In order to compare and analyze the β-glycosidase-producing abilities of wild yeasts and screen the high yield strains, 941 wild yeast strains isolated from the east foot of Helan mountain in Ningxia were screened through esculin colorimetry and p-nitrophenyl-β-D-glucopyranoside(p-NPG)coloration methods. Strains were classified and identified by the colonial morphology in WL medium and by sequence analysis of 26 S r DNA D1/D2 domain. Besides, the difference analysis of the β-glycosidase-producing ability in...
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