【摘要】 目的:研究旋毛虫(T.spiralis)对肠炎小鼠[三硝基苯磺酸(TNBS)或唑酮(OXZ)诱导]脾脏淋巴细胞中Th1/Th2水平的影响。方法:雌性BALB/c小鼠,随机分为50%乙醇对照组、TNBS(OXZ)诱导肠炎模型组、预先感染T.spiralis后诱导TNBS(OXZ)模型组,每组小鼠取材时保证6只以上。对各组小鼠脾脏淋巴细胞进行分离,采用流式细胞术观察TNBS(OXZ)诱导肠炎模型组和预先感染T.spiralis后诱导TNBS(OXZ)模型组。造模后3 d和7 d脾脏淋巴细胞中Th1/Th2水平的变化。结果:与模型组相比,预先感染T.spiralis后诱导TNBS肠炎第3天脾脏Th1/Th2比值未见明显下降(P>0.05),于第7天明显降低(P<0.05)。诱导OXZ肠炎模型后第3天及第7天小鼠脾脏Th1/Th2比值均明显低于T.spiralis干预组(P<0.05)。结论:针对TNBS肠炎模型,T.spiralis可能是通过诱导Th2及Tr1型免疫反应抑制模型小鼠过度的Th1型免疫而起到良好的干预作用。在T.spiralis对OXZ模型小鼠的干预性研究中,并无T.spiralis感染诱发的Th2型炎症反应加重同样以Th2升高为主的OXZ模型小鼠的病情。更多还原

【Abstract】 Objective: To study the levels of Th1 and Th2 in spleen lymphocyte of TNBS and OXZ colitis without or with Trichinella spiralis( T. spiralis) infection. Methods: Female BALB/c mice were randomly divided into 3 groups: 50% Ethanol,TNBS or OXZ,T. spiralis+TNBS or OXZ( at least 6 in each group when mice were killed). The ratio of Th1/Th2 lymphocyte in spleen was detected by FCM with three color intracellular cytokine staining. Results: In spleen lymphocyte of TNBS colitic mice with prior nematode infection,the ratio of Th1/Th2 were not significantly different compared with that seen in colitic mice without prior nematode infection on day 3 post-induction of colitis( P>0. 05),the ratio of Th1/Th2 was significantly lower than which on day 7 post-induction of colitis( P<0. 05). In spleen lymphocyte of OXZ colitic mice with prior nematode infection on days 3 and 7 post-induction of colitis,the ratio of Th1/Th2 was significantly higher than that seen in colitic mice without prior nematode infection( P < 0. 05). Conclusion: Infection of mice with T. spiralis distracts the mucosal immune system response from a Th1 response toward a protective Th2 response and up-regulate Tr1-cytokines with an attendant reduction in the severity of colonic damage and balance of Th1/Th2. Prior T. spiralis infection doesn’t reduce the severity of OXZ-induced colitis,but without aggravating colitis.更多还原