Objective: To investigate the mechanism of Exendin-4 regulating proliferation of pancreatic β cell line INS-1. Methods: After stimulation of Exendin-4,the proliferation rate of INS-1 cells was detected by Ed U method. The expression of Wnt5 a gene was detected by real-time semi-quantitative PCR and Western-blotting. The proliferation rate of INS-1 cells was evaluated by Ed U method after Wnt5 a gene was silenced by siRNA. Cocultured with Exendin-4 and recombinant Wnt5 a protein,the proliferation rate was ob...