【摘要】 为了探究葡萄糖饥饿对HeLa细胞形态与结构的影响,采用噻唑蓝法检测了细胞活力;用倒置显微镜、荧光显微镜和电子显微镜观察了细胞形态与结构的变化;采用激光共聚焦免疫荧光技术观察了细胞微丝与微管的分布.结果表明:葡萄糖饥饿能够抑制HeLa细胞增殖,破坏细胞骨架,改变细胞形态;使细胞皱缩、染色质凝集,出现凋亡小体,微丝微管解聚,表现出典型的凋亡特征,且细胞凋亡程度呈葡萄糖浓度依赖性和处理时间依赖性.总之,葡萄糖饥饿能抑制HeLa细胞活性,改变细胞形态,诱导细胞凋亡.更多还原

【Abstract】 It was investigated the effects of glucose deprivation on morphology and structure of HeLa cells. The cell viability was analyzed by MTT assay,and the cell morphology and structure were observed by inverted microscopy,fluorescence and electron microscope. Additionally,immunofluorescence analysis and confocal microscopy were performed to observe the distribution of microtubules and microfilaments. The results showed that glucose deprivation could obviously inhibit the growth of HeLa cells and induce changes of morphology by destroying their skeletons. The cells exhibited typical apoptotic phenotype,including cell shrinkage,chromatin condensation,apoptotic bodies appeared,the microtubules and microfilaments depolymerized. With loss of the glucose concentration and increase of processing time,the degree of apoptosis increased. Glucose starvation could inhibit HeLa cell viability,modify cell shape,induce cell apoptosis.更多还原