【摘要】 本文记述了从浙江省和湖北省落叶树根围采集的隶属于长针线虫属的中国2新记录种——Longidorus pius和L.diadecturus,通过修订的Loof&Chen(1999)多岐检索表确认,其鉴定代码分别为A56-B4-C3-D1-E1-F3-G1-H1-I1和A4-B23-C5-D3-F2-G2-H1-I1。L.pius的主要形态学特征为:体长中等(4.7~6.3 mm),虫体前端明显变细,唇区圆滑,与体驱间无缢缩,齿针长(130~143μm),尾短而圆。L.diadecturus的主要鉴别特征为:体长较短(3.2~4.6 mm),齿针长(108~120μm),导环位置相对靠后(至唇端距离为33~38μm),唇区宽,阴门的位置相对靠前等。系统发育研究表明:来自中国的L.pius r DNA中ITS1区序列与来自马其顿的模式种L.pius群体相应序列(AM743178)处于同一进化枝中,置信度达99%;L.diadecturus与来自加拿大的模式种L.diadecturus群体相应序列(AF511416)处于同一进化枝中,置信度也达99%。另外,来自中国的L.diadecturus 28S区序列与模式种L.diadecturus的序列(AY601584)处于同一进化枝中,置信度达99%,而L.pius和L.raskii(AJ549983)、Longidorus sp.FDL-2011(FR775757)属于同一分枝。L.pius和L.diadecturus这2个种在中国均系首次报道,本文提供了详细的形态学描述及系统发育关系的有关信息。更多还原

【Abstract】 Two new recorded species of genus Longidorus(L. pius and L. diadecturus) were recovered and collected from rhizosphere of deciduous trees in Hangzhou of Zhejiang Province and Wuhan of Hubei Province,China, respectively. Morphological identity was confirmed using the revised polytomous key of Loof & Chen,1999. The codes for identifying the species of L. pius and L. diadecturus are A56-B4-C3-D1-E1-F3-G1-H1-I1 and A4-B23-C5-D3-F2-G2-H1-I1, respectively. L. pius was characterized by medium body size(L=4.7-6.3 mm), anterior region abruptly tapering with rounded lip continuous from body profile, odontostyle long(130-143μm) and short rounded tail. Meanwhile, L. diadecturus could be distinguished by short body size(3.2-4.6 mm),short odontostyle(108-120 μ m), posterior position of guide ring(33-38 μ m), wide lip region and a relatively anterior vulva. Phylogenetically, the r DNA internal transcribed spacer 1(ITS1) region of L. pius from China showed 99% supported clade with L. pius topotype(AM743178) from Macedonia, and the similar relationship was obtained in L. diadecturus from China with 99% well supported clade to L. diadecturus type population(AF511416) from Canada. In addition, the 28 S region of L. diadecturus from China showed 99% supported clade with L. diadecturus type specimen(AY601584), whereas L. pius noticeably clustered to L. raskii(AJ549983) and Longidorus sp. FDL-2011(FR775757). The two species, L. pius and L. diadecturus were recorded for the first time in China, and the morphological description and phylogenetic relationships of the given species were provided in this study.更多还原