【摘要】 目的检测乙型肝炎病毒(HBV)受体蛋白在人外周血单个核细胞(PBMC)与肝细胞的表达情况,探讨HBV进入PBMC的可能途径,以期了解HBV感染PBMC的可能机制。方法用淋巴细胞分离液分离人PBMC。用RIPA提取人PBMC和肝细胞(肝脏肿瘤癌旁组织)总蛋白,采用Western blot以检测HBV受体蛋白在两种细胞的表达情况。结果 Western blot检测肝细胞HBV受体蛋白阳性,与hNTCP抗体反应条带位于(35~40)×103之间,与预期一致;人PBMC蛋白无此反应条带,即hNTCP检测阴性。结论 PBMC不表达肝细胞表面的HBV受体蛋白,病毒可能循非受体途径进入PBMC,或PBMC表面的HBV受体与肝细胞表面的HBV蛋白受体蛋白不同。关于HBV感染PBMC的具体机制尚需进一步研究。更多还原

【Abstract】 Objectives To explore the possible pathways by which hepatitis B virus(HBV)enters peripheral blood monocytes(PBMCs)and to understand the possible mechanisms by which HBV infects PBMCs by detecting the expression of HBV receptor in PBMCs and hepatocytes. Methods In order to detect the expression of HBV receptor in PBMCs and hepatocytes,PBMCs were isolated with Ficoll-Hypaque.Total protein from PBMCs and hepatocytes from liver tumor-adjacent tissues was extracted with RIPA and then assayed using a Western blot assay. Results A Western blot assay indicated that HBV receptor protein expression was positive in hepatocytes,and the reaction band with the hNTCP antibody was located between(35~40)×103,as expected.However,the same reaction band with the hNTCP antibody was not detected in PBMCs,and HBV receptor protein expression was negative. Conclusion PBMCs do not express HBV receptor protein,though it was found on the surface of hepatocytes,suggesting that HBV may enter PBMCs via non-receptor pathways or that HBV receptor on the surface of PBMCs differs from HBV receptor on the surface of hepatocytes.More research is needed to interpret the exact mechanism by which HBV infects PBMCs.更多还原