Objectives To express SUDV GP in a prokaryotic system and to develop recombinant protein-based indirect ELISA for antibody detection. Methods A fragment of SUDV GP was amplified with PCR and then cloned into the prokaryotic expression vector pET30 a(+).Expression of GP was induced with IPTG.The IPTG concentration and time required for induction were optimized.Expressed products were purified with His-Ni affinity chromatography,followed by SDS-PAGE and Western blotting.An indirect ELISA technique was devised...
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