【摘要】 目的探讨血红素加氧酶1（heme oxygenase, HO-1）过表达对人脊髓星形胶质细胞（humanastrocytesofspinalcord,HA-sp）缺氧/复氧损伤的保护作用。方法通过给予HA-sp氯化钴（250μl/L CoCl₂）刺激模拟缺氧,然后再去除CoCl₂刺激,建立细胞缺氧/复氧损伤模型。采用免疫印迹（westernblot, WB）方法,从蛋白水平上检测不同刺激条件下HA-sp中HO-1的表达。采用四甲基偶氮唑蓝（methylthiazolyldiphenyl-tetrazolium bromide, MTT）方法,分析单纯给予缺氧/复氧刺激后以及上调HO-1表达的同时给予缺氧/复氧刺激后HA-sp的存活率变化。结果缺氧/复氧刺激条件下,HA-sp中HO-1蛋白过表达。利用锌原卟啉（ZnPPIX）抑制缺氧/复氧刺激条件下HO-1过表达后,HA-sp在缺氧/复氧刺激条件下存活率明显降低（P<0.01）;与单纯给予缺氧/复氧刺激后HA-sp的存活率相比,用钴原卟啉（CoPP）上调HA-sp中HO-1表达的同时给予缺氧/复氧刺激,细胞存活率明显升高（P<0.01）。结论利用CoPP上调HA-sp中HO-1的表达,具有抗细胞缺氧/复氧损伤的保护作用。更多还原

【Abstract】 Objective To investigate the protective effect of overexpressed heme oxygenase-1（HO-1） in hypoxia-reoxygenation of human astrocytes of spinal cord（HA-sp）. Methods Hypoxiareoxygenation mode was established by oxygen provision after CoCl₂ stimulation. The expression of HO-1 under different stimulation was detected by Western blotting. MTT assay was used to examine the relative survival of cell under hypoxia-reoxygenation as well as after up-regulated HO-1. Results HO-1 was upregulated by hypoxia/re-oxygen stimulation. The relative survival rate of HA-sp under hypoxiareoxygenation was significantly decreased after HO-1 protein overexpression was inhibited by ZnPPIX（P<0.01）, while the relative survival rate of HA-sp which was pretreated by CoPP was significantly increased compared with that of cells under hypoxia-reoxygenation（P < 0.01）. Conclusion HO-1 overexpression by CoPP exerts protective effect on hypoxia-reoxygenation injury of HA-sp. The result of this study may offer new thought for the therapy of hypoxia-reoxygenation injury of human spinal cord in the future.更多还原