【摘要】 蛋白质氧连-N-乙酰氨基葡萄糖(O-GlcNAc)糖基化修饰,是一种重要的蛋白质翻译后修饰(PTM),广泛存在于细胞核和细胞质中。蛋白质O-GlcNAc糖基化修饰,由O-GlcNAc转移酶(OGT)和O-GlcNAc水解酶(OGA)共同维持细胞内糖基化水平稳定,动态调节细胞信号转导途径中多种酶的功能,在许多生命活动中发挥着重要调节作用,并与多种代谢性疾病,如妊娠期糖尿病等的发生密切相关。笔者拟就蛋白质O-GlcNAc糖基化的生物合成途径、检测方法的最新研究进展进行综述。更多还原

【Abstract】 Protein O-linked N-acetylglucosamine(O-GlcNAc)glycosylation modification is one of the most important contents of protein post translation modification(PTM).Most of the protein O-GlcNAc glycosylation modifications take place at cell nucleus and cytoplasm.During the process of protein O-GlcNAc glycosylation modification,O-linked N-acetylglucosaminyltransferase(OGT)and O-linked N-acetylglucosaminidase(OGA)together maintain the stability of intracellular glycosylation levels.Protein O-GlcNAc glycosylation modification can dynamically regulate the functions of various enzymes in cell signal transduction pathway,and it plays an important regulatory role in many life activities,and it is closely related to the occurrence of various metabolic diseases such as gestational diabetes.The authors intend to review the latest research progress of the biosynthetic pathways and detection methods of protein O-GlcNAc glycosylation.更多还原