【摘要】 目的:研究幽门螺杆菌(Helicobacter pylori,HP)感染对胃癌组织中多梳抑制复合物2(polycomb repressive complex 2,PRC2)和组蛋白H3K27me3表达的影响,探索HP感染在胃癌发生过程中的作用。方法:利用快速尿素酶检测、Giemsa染色和PCR方法检测复旦大学附属上海市第五人民医院2014年1月至2017年10月行胃癌手术的84例患者HP感染情况,应用免疫组织化学法检测胃癌和癌旁组织PRC2和H3K27me3蛋白的表达,分析两者表达与HP感染之间的相关性。结果:3种方法检测HP感染率分别为70.24%、61.90%和76.19%。PRC2家族成员果蝇zeste基因增强子同源物2(enhancer of zeste homolog 2,EZH2)、SUZ12、EED和H3K27me3蛋白在胃癌组织中的阳性表达率分别为79.76%(67/84)、77.38%(65/84)、40.48%(34/84)和69.05%(58/84),均显著高于癌旁组织(22.62%、34.52%、8.33%和14.29%,P<0.05)。临床资料分析表明,EZH2和H3K27me3蛋白在胃癌中的表达与淋巴结转移呈正相关。在HP阳性胃癌组织中,EZH2、SUZ12、EED和H3K27me3蛋白阳性表达率分别为89.06%(57/64)、84.38%(54/64)、46.88%(30/64)和82.81%(53/64),均显著高于HP阴性胃癌组织(50.00%、55.00%、20.00%和25.00%,P<0.05)。结论:HP感染与胃癌组织中PRC2和H3K27me3蛋白表达上调相关。更多还原

【Abstract】 Objective: To investigate the effects of Helicobacter pylori(HP) infection on polycomb repressive complex 2(PRC2) and H3 K27 me3 expression and to explore the role of HP infection in the development of gastric cancer. Methods: Fast urease tests, Giemsa staining, and polymerase chain reaction(PCR) assays were conducted to detect HP in specimens from 84 gastric cancer patients, who had received surgeries in the Fifth People’s Hospital of Shanghai from January 2014 to October 2017. Immunohistochemistry staining was applied to examine PRC2 and H3 K27 me3 expression in gastric cancer tissues and para-tumor tissues. The relationship between HP infection and PRC2 and H3 K27 me3 expression was analyzed. Results: The infection rates of HP were 70.24%, 61.90%, and 76.19% as detected by the three methods, respectively. The expression of enhancer of zeste homolog 2(EZH2), suppressor of zeste 12(SUZ12), embryonic ectoderm development(EED), and H3 K27 me3 protein were detected in 79.76%(67/84), 77.38%(65/84), 40.48%(34/84), and 69.05%(58/84) of gastric cancer tissues, respectively, all of which were significantly higher than those of para-tumor tissues(22.62%, 34.52%, 8.33%, and 14.29%,respectively; P<0.05). The expression of EZH2 and H3 K27 me3 were positively correlated with lymph node metastasis, and the expression of these two proteins were also positively correlated. In gastric cancer tissues with HP infection, the expression of EZH2, SUZ12, EED, and H3 K27 me3 protein were detected at rates of 89.06%(57/64), 84.38%(54/64), 46.88%(30/64), and 82.81%(53/64), respectively, which were significantly higher than in gastric cancer tissues without HP infection(50.00%, 55.00%, 20.00%, and 25.00%, respectively; P<0.05).Conclusions: HP infection is associated with increased expression of PRC2 and H3 K27 me3 proteins in gastric cancer.更多还原