Tandem expression for the main antigenic domain of the AP1,BP and AP2 ancillary protein in pilus insland of Streptococcus agalactiae and the identification of antigenicity
In order to investigate the antigenicity of AP1, AP2 and BP fusion protein in pilus island of Streptococcus agalactiae, primers were designed and synthesized based on the major antigenic domains of the 3 subunits genes. Nucleotide sequences of the 3 subunits were obtained by the SOE PCR technique, and cloned into pET-30 a(+) vector to construct recombinant plasmid. The recombinant plasmid was transformed into BL21(DE3) competent cells to expresse recombiont protein.Purified fusion protein was detected by we...
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