【摘要】 目的探讨高危型乳头瘤病毒(HPV)与P16、Ki-67检测在宫颈癌早期筛查中的价值。方法选择河源市人民医院2015年1月~2017年12月应用免疫组织化学方法检测30例宫颈正常组织、75例宫颈上皮内瘤变(CIN)中P16和Ki-67的表达,采用PCR法检测高危型HPV感染情况。结果宫颈上皮内低度瘤变(CINⅠ)中,P16阳性率为77.80%,Ki-67阳性率为65.80%,高于宫颈正常组织0.00%,差异有统计学意义(P<0.05);宫颈上皮内高度瘤变(CINⅡ~Ⅲ)中,P16和Ki-67阳性率为100.00%、94.70%,高于CINⅠ,差异有统计学意义(P<0.05)。高危型HPV在宫颈正常组织、CINⅠ和CINⅡ~Ⅲ病变组感染者分别为6.70%、66.70%、84.60%和90.30%,宫颈癌前病变组织明显高于对照组,差异有统计学意义(P<0.05)。结论高危型HPV感染及P16、Ki-67与宫颈癌前病变发生发展相关,联合应用可作为CIN分级诊断的辅助方法,有较好的应用价值。更多还原

【Abstract】 Objective To explore the value of high-risk HPV,P16 and Ki-67 detection in early screening of cervical cancer.Methods From January 2015 to December 2017,30 cases of normal cervical tissue and the expression of P16 and Ki-67 in 75 cases of cervical intraepithelial neoplasia(CIN) were detected by immunohistochemistry in our hospital.The high-risk HPV infection was detected by polymerase chain reaction(PCR).Results The positivity rates of P16(77.80%) and Ki-67(65.80%) and In the CIN Ⅰ,higher than those in normal cervical tissue accounting for 0.00%.The differences were statistically significant(P<0.05).The positivity rates of P16(100.0%) and Ki-67(94.70%) in CINⅡ-Ⅲwas higher than those of CINⅠ(P<0.05).High-risk HPV infection rates in normal cervical tissues,CINⅠ,and CIN Ⅱ-Ⅲwas 6.70%,66.70%,84.60% and 90.30% respectively,and the infection in the precancerous lesions the uterine cervix was significantly higher than that in the control group,the differences were statistically significant(P<0.05).Conclusion High-risk HPV infection,P16,and Ki-67 are associated with the occurrence and development of precancerous lesions of the uterine cervix.Use in combination can be considered as an auxiliary method for the diagnosis of CIN grading and has a good application value.更多还原