【摘要】 目的临床上建立一种快速、准确测定儿童血浆中维生素E含量的方法。方法取儿童血浆200μl,按v/v为8∶2的比例加入甲醇和异丙醇140μl,涡旋30 s,加入正己烷1 ml,涡旋1 min,在室温条件下高速离心120 s(12 000×g),取离心后上清液700μl,吹干,用100μl甲醇复溶。色谱条件:采用Waters公司BEH C18色谱柱(50 mm×2.1 mm,1.7μm);取处理好的溶液5μl进样,以0.3 ml/min的流速测定;甲醇-水(96∶4)为流动相;柱温为30℃;检测波长:291 nm。结果血浆中维生素E色谱峰分离良好,无干扰线性回归方程为:y=118296x+11644,r2=0.9997,回收率在90%~120%。结论本方法操作简便,适用于儿童血浆中维生素E含量的快速监测。更多还原

【Abstract】 Objective To establish a rapid and accurate method in the determination of the content of Vitamin E in children′s plasma clinically.Methods 200 μl children′s plasma was collected.140 μl methanol and isopropanol were added with the proportion of v/v,8:2,which were vortexed for 30 s.1 ml n-hexane was added and vortexed for 1 min.They were centrifuged at a high speed at room temperature for 120 s(12 000×g).700 μl supernatant was collected after centrifuge,blown dry and redissolved by 100 μl methanol.Chromatographic condition:BEH C18 chromatographic column from Waters company was applied(50 mm×2.1 mm,1.7 μm).The prepared solution of 5 μl was taken as a sample,and determined with the flow rate of 0.3 m L/min.Methanol-water(96:4) was the mobile phase.The column temperature was 30℃.Under the condition,the determination wavelength was 291 nm.Results The chromatographic peak of vitamin E in plasma was separated favorably,and non-interference linear regression equation was y=118296 x+11644,r2= 0.9997.The recovery rate was 90%-120%.Conclusion The method is simple to operate,which is suitable for the rapid determination of Vitamin E content in children′s plasma.更多还原