【摘要】 目的探讨糖尿病大鼠胃平滑肌细胞凋亡与哺乳动物雷帕霉素靶蛋(mTOR)及下游因子p70S6K、p-4E-BP1的变化及意义。方法制备糖尿病大鼠模型,分为正常对照(NC)组、模型2W(DM2W)组、模型4W(DM4W)组和模型6W(DM6W)组。流式细胞技术检测各组胃平滑肌细胞凋亡率,Western blot检测胃平滑肌p-mTOR、p-p70S6K、p-4E-BP1蛋白的表达。结果DM2W、DM 4W与DM 6W组胃平滑肌细胞细胞凋亡表现为逐渐增加,p-mTOR、p-p70S6K、p-4E-BP1表达均为先升高(与NC组比较,P<0.01)后降低(与DM4W组比较,P<0.01)。结论糖尿病大鼠胃平滑肌存在细胞凋亡与mTOR通路的变化,其中mTOR对细胞凋亡的调控不处于主导地位。更多还原

【Abstract】 Objective To explore the role and significance of the apoptosis and the mTOR Signal pathway,including p70S6 Kand p-4 E-BP1,in gastric smooth muscle of diabetic rats. Methods SD rats were divided into normal control group(NC group),model two weeks group(DM2 W group),model four weeks group(DM4W group)and model six weeks group(DM6W group).The apoptosis rate of gastric smooth muscle in each group was detected by flow cytometry.The expression of p-mTOR,p-p70S6 Kand p-4 E-BP1 in each group was evaluated by Western blotting. Results The apoptosis rates gradually increased in DM2W,DM4W and DM6W groups.The protein expression levels of p-mTOR,p-p70S6 Kand p-4 E-BP1 firstly increased(compared with the normal group,P<0.01)and then decreased(compared with 4W group,P<0.01). Conclusion The apoptosis and mTOR pathway changes are exist in gastric smooth muscle in diabetic rats.The effect of mTOR pathway is not dominant in apoptosis regulation.更多还原