【摘要】 观察β-乳球蛋白(β-Lg)过敏小鼠模型中miRNA的异常表达和Th17相关细胞因子分泌的相关性,同时研究嗜酸乳杆菌的干预作用,探究益生菌缓解牛乳过敏的分子机制。BALB/c小鼠随机被分为正常对照组,β-Lg过敏模型组和低、中、高剂量嗜酸乳杆菌干预组,以ELISA法检测过敏小鼠血清中Th17相关细胞因子的分泌,RT-PCR法检测过敏小鼠脾脏组织CD4+T细胞中与炎症相关的miRNA基因表达,并对两者进行相关性分析。在此基础上研究嗜酸乳杆菌干预过敏模型对miRNA表达的影响。与正常对照组比较,过敏组小鼠血清IgE,ILI7,IL-6,TNF-α及IL-21水平显著增高(P<0.05);其脾脏组织CD4+T细胞中miR-155,miR-21,miR-9和miR-146a mRNA表达量明显上调(P<0.05),与Th17细胞的RORγt及IL-17mRNA表达水平呈正相关(P<0.05)。嗜酸乳杆菌干预可显著下调过敏小鼠Th17细胞亢进,以及上述miRNA基因的异常表达(P<0.05),具有剂量依赖性。以上结果显示,miRNA在β-Lg过敏中发挥重要作用,嗜酸乳杆菌缓解过敏的作用可能与其调节miRNA表达密切相关。更多还原

【Abstract】 The present study was planned to analyze the correlations between Th17-related cytokine production and abnormal miRNA expression in allergic mouse model, and investigate the intervention effect of L. acidophilus in this process. BALB/c mice were randomly divided into normal control group, β-Lg allergy group, and L. acidophilus group(low, medium high dose). After the allergen challenge, the levels of serum Th17-related cytokine were measured by ELISA, the miRNA expression in spleen tissue CD4+T cells were measured by real-time PCR, and made the correlation analysis. Furthermore,the effect of L. acidophilus on the miRNA expression were analyzed. The results showed that sensitization with β-lg led to develop the allergic response, the serum IgE, IL-17, IL-6, TNF-α and IL-21 expression were increased in β-lg-sensitized mice compared to the controls(P<0.05). In addition, the miR-155, miR-21, miR-9 and miR-146 a mRNA expression of β-lg-sensitized mice were positively correlated with IL-17 and RORγt mRNA( P <0. 05). Compared to allergic mice, oral administration of L. acidophilus suppressed hypersensitivity responses, decreased the expression of IL-17 and RORγt mRNA, together with inhibited abnormal miRNA expression in a dose-dependent manner. These findings suggested that L. acidophils could regulate miRNA expression, which may be responsible for the suppression of allergic symptoms.更多还原