【摘要】 目的本研究探讨白藜芦醇对缺氧诱导胃癌细胞的上皮间质转化(epithelial-mesenchymal transition,EMT)的抑制作用及其可能机制。方法在缺氧和正常情况下培养胃癌细胞SGC-7901,设立对照组、白藜芦醇组、缺氧组、白藜芦醇+缺氧组,通过四甲基偶氮唑盐比色(MTT)法检测白藜芦醇对胃癌细胞的增生抑制作用,细胞划痕试验检测白藜芦醇对胃癌细胞侵袭转移的抑制作用,荧光定量PCR(RT-qPCR)、蛋白免疫印迹法(Western Blot)检测胃癌细胞中上皮间质转化相关因子Snail、E-cadherin及vimentin的表达情况。结果与对照组相比,缺氧状态下胃癌细胞SGC-7901的侵袭和转移能力显著增强,缺氧状态下,E-cadherin的表达降低,而Snail和vimentin的表达升高,白藜芦醇则可以抑制缺氧的影响,且白藜芦醇的抑制作用主要表现为对HIF-1α蛋白表达的影响,而对HIF-1α mRNA无影响。结论白藜芦醇可能通过影响HIF-1α蛋白的表达,抑制胃癌细胞的增生、侵袭及上皮间质转化过程。更多还原

【Abstract】 Objective To explore the inhibitive effect and its possible mechanism of resveratrol on epithelial-mesenchymal transition(EMT) of gastric carcinoma cells induced by hypoxia.Me thods Gastric carcinoma SGC-7901 cells were cultured under hypoxic condition and normal conditions.The control group,the resveratrol group,the hypoxia group and resveratrol+ hypoxia group were established.The methabenzthiazuron(MTT) assay was used to evaluate the inhibitive effect on proliferation of SGC-7901 cells.The wound scratch assay was adopted to determine the inhibitive effect of resveratrol on the migration ability of gastric carcinoma cells.PCR(RT-qPCR) and Western Blot analysis were used to detect the expression of epithelial-mesenchymal transition related factors in gastric cancer cellssnail,such as E-cadherin and vimentin protein levels.Re s ults Compared with the control group,hypoxia significantly increased the proliferation rate and invasive ability of SGC-7901 cells.Moreover,hypoxia markedly decreased the E-cadherin level and increased Snail and vimentin expression.However,resveratrol reversed all the above effects of hypoxia in SGC-7901 cells.Moreover,resveratrol inhibited HIF-1α protein accumulation without affecting the HIF-1α mRNA level.Conclus ion Resveratrol could inhibit the proliferation,invasion and epithelial-mesenchymal transition process in gastric carcinoma cells via affecting the accumulation of the HIF-1α protein.更多还原