【摘要】 目的检测皮肌炎(DM)和类风湿性关节炎(RA)伴间质性肺疾病(ILD)患者支气管肺泡灌洗液(BALF)中骨膜蛋白的表达及临床意义。方法对44例DM-ILD和28例RA-ILD患者进行支气管肺泡灌洗,回收BALF,检测BALF中骨膜蛋白和涎液化糖链抗原-6(KL-6)水平,并对BALF进行细胞分类计数。对每位患者进行高分辨率CT评分。另选30例无ILD病变的患者作为对照组。结果 DM-ILD和RA-ILD患者BLAF中骨膜蛋白、KL-6水平均明显高于对照组(均P<0.05)。骨膜蛋白水平与BALF中淋巴细胞计数、KL-6水平均呈正相关(均P<0.05)。骨膜蛋白与DM-ILD、RA-ILD患者高分辨率CT评分呈正相关(P<0.05)。结论 DM-ILD和RA-ILD患者BALF中骨膜蛋白水平升高,是CTD-ILD的潜在预测指标。更多还原

【Abstract】 Objective To explore the expression of periostin in bronchoalveolar lavage fluid(BALF) of patients with dermatomyositis-related interstitial lung disease(DM-ILD) and rheumatoid arthritis-related interstitial lung disease(RA-ILD). Methods A total of 44 patients with DM-ILD and 28 patients with RA-ILD were underwent bronchoalveolar lavage. Cells in BALF were collected and analyzed by absolute different cell counts. The level of periostin and Krebs von den Lungen-6(KL-6) were tested by enzyme linked immunosorbent assay. Results of high resolution CT of patients were scored. Thirty patients without interstitial lung disease(ILD) served as a control group. Results Levels of periostin and KL-6 were significantly increased in BALF of patients with DM-ILD and RA-ILD compared with control group(all P<0.05). Levels of periostin were positively correlated with lymphocyte counts and levels of KL-6 in BALF(all P<0.05).Furthermore, levels of periostin were significantly correlated with high resolution CT scores(P<0.05). Conclusions Levels of periostin are increased in patients with DM-ILD and RA-ILD. Periostin might be served as an indicator of CTD-ILD.更多还原