【摘要】 目的研究七氟醚对新生大鼠神经干细胞增殖影响。方法取出生7 d的SD大鼠48只,体重15~20 g,随机分3组:对照组(Con组)、1%七氟醚组(S1组)、3%七氟醚组(S2组)。S1组和S2组分别吸入1%、3%七氟醚4 h。各组新生鼠麻醉开始前腹腔注射5-溴脱氧尿苷100 mg/kg,于麻醉结束后6 h处死大鼠并分离脑室区和脑室下区组织。采用免疫组化法进行Nestin与Brd U染色,计算检测脑室区和脑室下区神经干细胞增殖率;Western blot法检测Nestin、Cyclin D1、BMP2、Smad4蛋白的表达。结果与对照组比较,S1组和S2组大鼠脑室区和脑室下区组织Nestin蛋白表达降低、神经干细胞增殖率降低,Cyclin D1表达下降,BMP2、Smad4表达上调(P<0.05)。结论七氟醚可抑制新生大鼠神经干细胞的增殖,其机制可能与Cyclin D1、BMP2、Smad4蛋白表达变化有关。更多还原

【Abstract】 Objective To evaluate the effect of sevoflurane on the proliferation of neural stem cells( NSCs) in the ventricular/subventricular zone of neonatal rats. Methods Forty-eight 7-day-old Sprague-Dawley rats,weighing 15-20 g,were divided into three groups( n = 16 each) using a random number table: control group( Con),1% sevoflurane group( S1),and 3% sevoflurane group( S2). Rats in the control group inhaled air for 4 h. Rats in S1 and S2 groups inhaled 1% and 3% sevoflurane,respectively,for 4 h. Cell proliferation was detected by Nestin and 5-bromodeoxyuridine staining. Protein expression of Nestin,Cyclin D1,BMP2,and Smad4 in ventricular/subventricular tissues was analyzed by Western blotting. Results Compared with the control group,the proliferation rate of NSCs was decreased significantly.Expression levels of Nestin and Cyclin D1 were downregulated,but expression levels of BMP2 and Smad4 were significantly upregulated after sevoflurane treatment( P < 0. 05). Conclusions Sevoflurane inhibits proliferation of NSCs in the ventricular/subventricular zone of neonatal rats and the mechanism may be related to regulation of Cyclin D1,BMP2,and Smad4 expression.更多还原