【摘要】 目的探讨KLF2转录因子在RDS大鼠肺组织的表达以及与甲状腺素水平的关系,为肺泡表面活性物质治疗不敏感的NRDS提供可能的理论依据。方法注射油酸建立的RDS大鼠模型为研究组,分别于注射后3 h、18 h、24 h用电化学发光法检测血清甲状腺素水平,用Western blot法检测肺组织中KLF2蛋白的表达,并与非RDS大鼠做对照。结果油酸3 h组、18 h组中的血清FT4与正常对照组比较差异有统计学意义(P<0. 01);油酸3 h组中T4与正常对照组比较差异有统计学意义(P<0. 01);油酸18 h组中T4与正常对照组比较差异有统计学意义(P<0. 05)。肺组织中的KLF2蛋白量表达油酸3 h组与正常对照组比较差异有统计学意义(P<0. 05),而18 h组、24 h组与正常对照组差异无统计学意义(P>0. 05)。结论呼吸窘迫综合症与血清甲状腺素水平、KLF2蛋白的表达有相关性,FT4、T4与KLF2有相关性。更多还原

【Abstract】 Objective To investigate the expression of KLF2 transcription factor in lung tissue of RDS rats and its relationship with thyroxine level. Methods The RDS rats models( study group) induced by injecting acid were detected the serum level of thyroxine by Electrochemiluminescence after injection 3 h,18 h,24 h,and the expression of KLF2 protein in lung tissue was tested by Western blot,non-RDS rats were as contral group. Results The serum levels of FT4,T4 were significant differences between 3 h,18 h study groups and normal groups( P<0. 01,P<0. 05). The expression of KLF2 protein in lung tissue was significantly different between the 3 h study group and control group,and there were no significant differences between the 18 h,24 h study groups and control groups. Conclusion Respiratory distress syndrome is related to the serum levels of thyroxine and KLF2 protein,and FT4,T4 is related to KLF2.更多还原