【摘要】 目的评价MicroDST^TM微孔板药敏（简称微孔药敏）检测法对结核分枝杆菌对一、二线抗结核药物敏感性试验的临床价值。方法采用随机数字表法选取BACTEC MGIT 960快速培养法培养的201株并经鉴定为结核分枝杆菌的分离株,采用微孔药敏法对一、二线抗结核药物,包括链霉素（SM）、异烟肼（INH）、利福平（RFP）、乙胺丁醇（EMB）、阿米卡星（AM）、卷曲霉素（CM）、左氧氟沙星（LFX）、莫西沙星（MFX）、对氨基水杨酸（PAS）、丙硫异烟胺（PTO）对分离株进行药物敏感性检测,并将其结果与罗氏比例法药敏检测结果进行比较分析,评价两种方法的符合率。结果 201株结核分枝杆菌临床分离株用微孔药敏法进行药敏检测的结果判读时间为7～14 d,其结果与"金标准"的罗氏比例法药敏的结果比较,微孔药敏检测的敏感性均不低于80%,且链霉素（SM）、异烟肼（INH）、乙胺丁醇（EMB）、利福平（RFP）、左氧氟沙星（LFX）、莫西沙星（MFX）、阿米卡星（AM）、卷曲霉素（CM）、对氨基水杨酸（PAS）、丙硫异烟胺（PTO）、的符合率分别为96.2%、92.2%、92.2%、93.8%、85.7%、94.1%、93.3%、84.6%、94.1%、100%。结论微孔药敏方法检测MTB对一、二线抗结核药物敏感性的检测结果与罗氏比例法检测的药敏结果具有较高的一致性,且检测快速,易于推广,符合了当前结核病的诊断需求。更多还原

【Abstract】 Objective To evaluate the clinical signification of the MicroDST^TMin detecting the susceptibility of clinical Mycobacterium tuberculosis isolates to the first and second line anti-tuberculosis drugs.Method Cultured the Mycobacterium tuberculosis by BACTEC TM CMGITTM 960 method,201 plants were selected from microbiology laboratory of shaanxi provincial tuberculosis control hospital by random number table method. Detected the sensitivity of streptomycin（ SM）,isoniazid（ INH）,rifampicin（ RFP）,ethambutal（ EMB）, amikacin（ AM）, capreomycin（ CM）, levofixacin（ LFX）, moxifloxacin（ MFX）, paraaminosalicylic（ PAS）,protionamide（ PTO） by MicroDST^TM. Meanwhile,the drug susceptibility results were compared with the ratio of Roche to the drug sensitivity test. Result By MicroDST^TM,the best results were read between 7 to 14 days. The sensitivity was compared with the Roche drug susceptibility text not less than80%. And the coincidence rate of streptomycin（ SM）,isoniazid（ INH）,rifampicin（ RFP）,ethambutal（ EMB）,amikacin（ AM）,capreomycin（ CM）,levofixacin（ LFX）,moxifloxacin（ MFX）,para-aminosalicylic（ PAS）,protionamide（ PTO） were 96. 2%,92. 2%,92. 2%,93. 8%,93. 3%,85. 7%,84. 6%,94. 1%,94.1%,100%. Conclusion The result of the drug Microporous susceptibility test by the MicroDST^TMmethod was highly consistent with the ratio of Roche to the drug sensitivity test,and the former more rapid than the latter. Therefore,MicroDST^TMmethod can satisfy clinical demand.更多还原