【摘要】 目的 :探讨外周血淋巴细胞中G蛋白偶联受体激酶5(G protein-coupled receptor kinase-5,GRK5)水平与阿尔茨海默病(Alzheimer′s disease,AD)患者认知障碍间的相关性。方法:选择2015年1月至2017年12月期间我院住院和门诊的AD患者,根据简易智能精神状态量表(mini-mental state examination,MMSE)评分分为轻度AD(27例)和中-重度AD组(37例),另选择30名健康体检者作为对照。应用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测所有研究对象外周血淋巴细胞中的GRK5蛋白表达情况,进行组间比较,并分析外周血GRK水平与患者疾病间的相关性。结果:轻度[(7.58±0.50)ng/m L]及中-重度AD组[(4.37±0.26)ng/m L]外周血淋巴细胞中GRK5水平与对照组[(15.65±0.74)ng/m L]相比,差异均有统计学意义(P<0.001);轻度AD组与中-重度AD组相比,差异亦有统计学意义(P<0.001);线性回归分析显示,外周血淋巴细胞中GRK5蛋白水平与MMSE评分呈显著正相关(R2=0.678 1)。结论 :AD患者存在GRK5水平下降;外周血淋巴细胞GRK5可能作为早期诊断AD的一种新型生物标志物。更多还原

【Abstract】 Objective: To investigate the correlation between G protein-coupled receptor kinase-5(GRK5) level in peripheral lymphocytes and the degree of cognitive impairment in Alzheimer’s disease(AD) patients. Methods: A total of64 patients with AD during January 2015 to December 2017 were enrolled and divided into two groups, including 27 cases of mild dementia and 37 cases of moderate-to-severe dementia; 30 healthy subjects were served as controls. Enzyme-linked immunosorbent assay(ELISA) was used to detect the GRK5 level in peripheral lymphocytes of AD patients and controls.Results: The GRK5 level in mild and moderate-to-severe AD patients was significantly lower than that in controls(P <0.001). Moreover, the GRK5 level in moderate-to-severe AD patients was significantly lower than that in the mild AD patients(P <0.001). Linear regression analyses revealed a strong positive correlation between the GRK5 level and the Mini Mental State Examination(MMSE) score(P <0.001). Conclusions: Our data suggestes that low level of GRK5 is existed in AD patients and mirrored the impairment of cognitive state. GRK5 level of peripheral blood lymphocytes might be used as a novel biomarker for the early diagnosis of AD patients.更多还原