【摘要】 目的建立运用超高效液相色谱-串联四极杆质谱联用技术同时测定西洋参花蕾中4种皂苷类成分(人参皂苷Re、拟人参皂苷F₁₁、人参皂苷Rb₃、人参皂苷Rd)含量的分析方法。方法采用Waters Acquity UPLC BEH C₁₈（50 mm×2.1 mm,1.7μm）色谱柱;以乙腈和0.05%氨水为流动相进行梯度洗脱;体积流量为0.45 mL/min;柱温35℃;采用电喷雾离子源（ESI）负离子检测方式,多反应监测模式（MRM）。结果该分析方法的精密度、稳定性、重复性和加样回收率考察结果均符合要求,各对照品在相应的浓度范围内,线性关系良好。结论建立的方法快速,简便,准确度高,可靠性强,可用于西洋参花蕾中皂苷类成分的分析。更多还原

【Abstract】 Objective To establish the determination method of four ginsenosides from flower buds of Panax quinquefolium by Waters Acquity UPLC H-Class with Xevo TQD. Methods The chromatographic separation was performed on a Waters Acquity UPLC BEH C18 column(50 mm × 2.1 mm, 1.7 μm). The mobile phase was a mixture of acetonitrile and water containing 0.05% ammonium hydroxide with gradient elution; The flow rate was 0.45 m L/min, and the column temperature was 35 ℃; Multiple reaction monitoring(MRM) acquisition under negative ion scan mode by ESI was also performed. Results The method was established with good precision, stability, repeatability, and accuracy. Ginsenoside Re, pseudo-ginsenoside F11, ginsenoside Rb3, and ginsenoside Rd showed a good linearity in the range of corresponding concentrations. Conclusion The UPLC-MS/MS method is rapid, simple, accurate, reliable, which can be used for the analysis of ginsenosides from flower buds of P. quinquefolium.更多还原