【摘要】 目的优化猴头菌素A 2种包合物制备工艺,并评价其稳定性。方法以投料比、研磨时间、研磨速度为影响因素,包合率、收率为评价指标,正交试验优化制备工艺。红外分光光度法和TLC法对所得包合物进行表征,并考察其在高温(60℃)、强光(3 000 lx)、高湿度[(90±5)%]下的稳定性。结果环糊精最佳包合条件为投料比5∶1,研磨时间60 min,研磨速度300 r/min,包合率20.66%,收率88.21%;羟丙基-β-环糊精最佳包合条件为投料比25∶1,研磨时间90 min,研磨速度400 r/min,包合率69.25%,收率96.31%。猴头菌80%乙醇提取物的包合是物理过程,未发生成分变化。在高温、强光下,两种包合物外观无明显变化,猴头菌素A损失小,在高湿度条件下恰好相反。羟丙基-β-环糊精包合物的包合效果优于β-环糊精包合物。结论猴头菌素Aβ-环糊精、羟丙基-β-环糊精包合物具有良好的热稳定性、光稳定性,但易发生潮解。更多还原

【Abstract】 AIM To optimize the preparation of two inclusion compounds of erinacine A and to evaluate their stabilities. METHODS With feed ratio,milling time and milling speed as influencing factors,inclusion rate and yield as evaluation indices,the preparation was optimized by orthogonal test. The obtained inclusion compounds were characterized by infrared spectrophotometry and TLC,whose stabilities at high temperature( 60 ℃),strong light( 3 000 lx) and high humidity [( 90 ± 5) %]were investigated. RESULTS The optimal β-cyclodextrin inclusion conditions were determined to be 5 ∶ 1 for feed ratio,60 min for milling time,and 300 r/min for milling speed,the inclusion rate and yield were 20. 66% and 88. 21%,respectively. The optimal hydroxypropyl-β-cyclodextrin inclusion conditions were determined to be 25 ∶ 1 for feed ratio,90 min for milling time,and 400 r/min for milling speed,the inclusion rate and yield were 69. 25% and 96. 31%,respectively. The inclusion of Hericium erinaceus 80% ethanol extract was physical process without composition change. At high temperature and strong light,two inclusion compounds’ appearance showed no obvious change with little loss of erinacine A,which was just the contrary at high humidity. The inclusion effect of hydroxypropyl-β-cyclodextrin inclusion compound was better than that of β-cyclodextrin inclusion compound. CONCLUSION Both β-cyclodextrin and hydroxypropyl-β-cyclodextrin inclusion compounds of erinacine A exhibit good heat stability and light stability,but deliquescence can easily happen to them.更多还原