【摘要】 目的研究牛磺酸对7,12-二甲基苯蒽(7,12-dimethyl benzene[a]anthracene,DMBA)诱发大鼠乳腺癌的干预作用及其机制。方法将30只6周龄雌性SD大鼠随机分为牛磺酸干预组、模型对照组和空白对照组,每组10只。牛磺酸干预组和模型对照组大鼠给予15 mg/100g的DMBA,在牛磺酸干预组的日常饮水中添加3%牛磺酸,一次性灌胃;空白对照组大鼠予1 m L/100 g花生油灌胃。所有大鼠均自由饮水和进食。观察、记录大鼠乳腺肿瘤发生情况,采用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测大鼠血清IL-6和TNF-α含量。结果与模型对照组相比,牛磺酸干预组肿瘤发生的潜伏期延长,致瘤率、荷瘤总数、血清IL-6和TNF-α水平均下降,差异有统计学意义(P<0.05),肿瘤体积和重量增加,但差异无统计学意义(P>0.05);与空白对照组相比,模型对照组血清IL-6和TNF-α水平显著升高[(41.41±10.10)pg/m L vs(20.00±10.10)pg/m L,P<0.05;(71.72±25.83)pg/m L vs(42.00±16.69)pg/m L,P<0.05),而牛磺酸干预组升高不明显[(22.48±10.38)pg/m L vs(20.00±10.10)pg/m L,P>0.05;(42.52±9.15)pg/m L vs(42.00±16.69)pg/m L,P>0.05)。结论牛磺酸对DMBA诱发大鼠乳腺癌的发生有较明显的抑制作用,其机制可能通过调节机体免疫反应途径实现。更多还原

【Abstract】 Objective To study the effects of taurine on 7,12-dimethyl benzene[a]anthracene(DMBA)-induced breast cancer in rats and its mechanism of action. Methods A total of 30 male Sprague-Dawley rats were randomly divided into three groups:taurine intervention group,model control group and blank control group. Rats in taurine intervention group and model control group were given DMBA(15 mg/100 g)by one-time gavage,while rats in blank control group were given peanut oil(1 m L/100 g). Animals in taurine intervention group also received 3% taurine in their drinking water. All rats could drink water and eat ad libitum. Levels of IL-6 and TNF-α in serum were measured using enzyme-linked immunosorbent assay(ELISA). Results Compared with model control group,taurine intervention group showed a lower rate of tumorigenesis,longer tumor latency,and smaller total number of tumors(P<0.05). Tumor volume and mouse body weight were similar between taurine intervention group and model control group(P >0.05). Levels of serum IL-6 and TNF-α were significantly lower in taurine intervention group than in model control group(P<0.05),significantly higher in model control group than in blank control group[(41.41 ±10.10)pg/m L vs(20.00±10.10)pg/m L,P<0.05;(71.72±25.83)pg/m L vs(42.00±16.69)pg/m L,P <0.05)],and similar between taurine intervention group and blank control group [(22. 48 ± 10. 38)pg/m L vs(20. 00 ± 10. 10) pg/m L,P >0.05;(42.52±9.15) pg/m L vs(42.00±16.69) pg/m L,P>0.05)]. Conclusions Taurine has a significant inhibitory effect on the development of DMBAinduced breast cancer in rats. Taurine may exert these effects by regulating the body’s inflammatory and immune responses.更多还原