【摘要】 番茄(Solanum lycopersicum L.)基因组中已鉴定到121个R2R3-MYB转录因子,但其中绝大多数的生物学功能仍然未知。为探究R2R3-MYB转录因子基因SlMYB102的功能,从‘Micro-Tom’番茄中,利用PCR技术克隆了SlMYB102的编码区,全长为1 089 bp。保守结构域和系统进化树分析显示,SlMYB102蛋白包含两个保守的MYB结构域,与马铃薯的StMYB34同源性最高。亚细胞定位结果显示,SlMYB102编码的蛋白定位于细胞核中。SlMYB102在根、茎、叶、花和未绿熟、红熟果实中均有表达,在茎、叶、花中表达量较高。利用农杆菌介导,将35S::SlMYB102转入番茄,获得了两个过表达株系。与野生型相比,过表达株系种子萌发速率明显升高,而植株高度、叶面积、地上部及地下部的鲜质量均显著降低。显微观察结果表明,与野生型相比,过表达株系叶片表皮细胞面积大小并没有变化,推测过表达SlMYB102是通过减少细胞分裂来抑制番茄植株的生长。更多还原

【Abstract】 A total of 121 R2R3-MYB transcription factors were identified in the tomato(Solanum lycopersicum L.)genome. But the biological function for most of these transcription factors was not clear. To explore the function of SlMYB102,its coding region of 1 089 bp was cloned from’Micro-Tom’tomato(WT)by PCR. Conserved domain and phylogenetic analysis showed that Sl MYB102 included two conserved domanins of MYB and shared the highest similarity with StMYB34. Subcellular localization analysis indicated that the protein encoded by SlMYB102 was located in the nucleus. SlMYB102 was expressed in all tissues,and relatively higher expression level was observed in stem,leaf and flower. Two transgenic tomato lines overexpressing SlMYB102 were obtained through Agrobacterium-mediated transformation. The germination rate of the seeds from the transgenic lines was faster than that from WT,while the plant height,leaf area,fresh weight of shoot and root were all decreased. However,the leaf epidermal cell size did not change in the transgenic lines compared with WT. Therefore,the overexpression of SlMYB102 may negatively regulate plant growth by reducing cell division in tomato.更多还原