Using scales and embryogenic callus as the target tissues,Lilium pumilum DC. Fisch. and Lilium davidii var. unicolor were accomplished the ployploid inducement by soaking and mixed culture with different concentrations of colchicine. Nineteen tetraploids of Lilium pumilum DC. Fisch. and 6 tetraploids of Lilium davidii var. unicolor have been identified,through morphological observation,root tip chromosome count and stomatal observation. The optimum condition is immersing embryogenic callus with 0.1% colchic...
原产于中国的细叶百合(Lilium pumilum DC.Fisch.)和兰州百合(Lilium davidii var.unicolor),具有较高的观赏价值和较强的抗逆性,但二者茎秆纤弱、叶片细狭、花朵娇小、结实率低等(Pitschmann et al.,2013)。在植物进化史中,多倍体化扮演着重要的角色(Sattler et al.,2016),是?