【摘要】 以菊花(Chrysanthemum morifolium)免疫白色锈病品种‘C029’为试验材料,基于白色锈病病原菌诱导的菊花转录组数据库,采用RACE(rapid-amplification of c DNA ends)方法克隆得到菊花DREB(Dehydration responsive element binding protein)基因,命名为CmDREBa-2。对其进行生物信息学分析和表达分析,结果表明:CmDREBa-2开放阅读框(ORF)全长291 bp,编码96个氨基酸;预测CmDREBa-2蛋白相对分子质量为11 159.04,p I为11.34,是一个定位于细胞核、亲水的不稳定蛋白;氨基酸序列和结构分析显示该蛋白含有1个由49个氨基酸残基组成的AP2保守结构域,所以该蛋白属于AP2/EREBP家族;进化树分析表明Cm DREBa-2属于DREB家族的A-1组,CmDREBa-2蛋白与菊花的其他两个DREB类蛋白Cm DREBa和CmDREBb的同源性较高;菊花‘C029’接种白色锈病病原菌6 h后,CmDREBa-2表达量达到最高,约为未接菌对照的34倍;用水杨酸(SA)、茉莉酸甲酯(Me JA)和乙烯利(ETH)处理‘C029’菊花叶片,结果表明CmDREBa-2受3种激素的诱导表达。更多还原

【Abstract】 Based on the information of transcriptome database derived from‘C029’,a Puccinia horiana Henn. immune Chrysanthemum morifolium cultivar,the c DNA sequence of DREB(Dehydration responsive element binding protein)was cloned. This DREB gene was named CmDREBa-2. Sequence analysis showed that the open reading frame(ORF)of CmDREBa-2 was 291 bp,encoding 96 amino acids. The molecular weight of the predicted protein was 11 159.04 and the p I value was 11.34. The protein was located in the nucleus,and it was a hydrophilic and unstable protein. The transcription factor of CmDREBa-2 contained the AP2 DNA binding domain which was composed by 49 amino acids. The results of phylogenetic analysis showed that the CmDREBa-2 belonged to the DREB family of A-1 and it had a close relationship with CmDREBa and Cm DREBb from C. morifolium. The expression level of CmDREBa-2 reached a maximum at 6 h after Puccinia horiana Henn. infection,with 34 times as much as the control. We also found that the expression of CmDREBa-2 was induced by SA,Me JA and ETH.更多还原