【摘要】 目的优化半枝莲中总黄酮的提取工艺。方法以醇浓度、料液比、提取时间为自变量,总黄酮提取量为因变量,通过因变量对自变量各水平的多元线性回归和二项式方程的拟合,结合效应面法优选总黄酮的提取工艺条件。结果确定最佳提取工艺条件为69.15%乙醇回流提取2次,料液比为1∶30.32,提取时间为120 min,提取率可达3.0%。结论采用星点设计-效应面法确定半枝莲中总黄酮的提取工艺条件,方法简便、可行,预测效果好。更多还原

【Abstract】 Objective To optimize the extracting process of total flavonoids from Scutellaria barbata D.Don.Methods The independent variables were alcohol concentration,extracting time and liquid-to-solid ratio.The dependent variable was extraction content of total flavonoids.Linear or nonlinear models were used to estimate the relationship between independent and dependent variables.Central composite design and response surface methodology(CCD-RSM) was used to optimize the process of extraction.Results Analysis indicated that the optimum conditions were 69.15% ethanol,solid liquid ratio 1∶30.32,extracting time120 min and 2 times for extraction.Extractive rate was 3.0%.Conclusion The extracting technology is simple,convenient and highly predictive.更多还原