【摘要】 目的建立胃炎灵颗粒的质量标准。方法采用薄层色谱(TLC)法鉴别胃炎灵颗粒中的黄芪、黄连、甘草;采用高效液相色谱(HPLC)法同时测定胃炎灵颗粒中栀子苷和芍药苷的含量。色谱柱:Diamonsil C18(4.6 mm×250 mm,5μm),流动相:甲醇-水(20∶80,V/V),流速:1 mL/min;检测波长:235 nm;柱温:25℃。结果TLC斑点清晰,分离度好,阴性对照无干扰;栀子苷、芍药苷分别在1.030～10.300μg、1.032～10.320μg范围内线性关系良好,平均加样回收率分别为98.92%、97.75%,RSD分别为1.39%、1.19%。结论该方法简便可行、可重现性好、专属性强,可用于胃炎灵颗粒的质量控制。更多还原

【Abstract】 Objective To establish the quality standard of Weiyanling Granules. Methods Astragali Radix, Coptidis Rhizoma, Glycyrrhizae Radix et Rhizoma in Weiyanling Granules were identified by thin-layer chromatography(TLC).The contents of geniposide and paeoniflorin in Weiyanling Granules were determinated by high performance liquid chromatography(HPLC) simultaneously. The HPLC method was established with Diamonsil C18 column(4.6 mm×250 mm,5 μm). The mobile phase was consisted of methyl alcohol-water(20∶80, V/V) with the flow rate of 1 mL/min. The detective wavelength was set at 235 nm, and the column temperature was at 25℃. Results TLC spots were clear and well-separated without interference from negative sample. The linear range of geniposide and paeoniflorin were 1.030-10.300 μg and 1.032-10.320 μg with the average recovery being 98.92% and 97.75%, RSD being 1.39% and 1.19%,respectively. Conclusion The method is simple, feasible, reproducible and accurate, and is suitable for the quality control of Weiyanling Granules.更多还原