【摘要】 目的探究CD151对结肠癌中Wnt信号通路变化的影响。方法采用Western blot方法分别检测正常结肠癌细胞(HT29)、CD151基因敲除的结肠癌细胞(CD151--HT29)中CD151、LRG-1以及LGR-5蛋白的表达情况;采用实时定量PCR技术分别检测HT29、CD151--HT29细胞中LRG-1以及LGR-5基因的表达情况;考察HT29、CD151--HT29细胞的侵袭能力。结果HT29细胞中存在明显的CD151、LRG-1以及LGR-5蛋白表达,而CD151--HT29细胞中并无CD151蛋白的表达,且可以观察到LRG-1以及LGR-5蛋白表达量明显下降;实时定量PCR结果显示,与HT29细胞比较,CD151--HT29细胞中的LRG-1以及LGR-5基因表达显著下降(P<0.05);细胞侵袭实验可见,HT29细胞侵袭能力强于CD151--HT29细胞(P<0.05)。结论 CD151基因的缺失能够抑制Wnt信号通路的起始蛋白LRG-1、LGR-5的表达,并且能够下调癌细胞侵袭能力,提示CD151基因对Wnt信号通路存在一定调控作用,这为结肠癌联合靶向治疗提供了一定的科学指导。更多还原

【Abstract】 Objective To explore the effect of CD151 on the changes of Wnt signaling pathway in colon cancer.Methods Western blot method was used to detect colon cancer cells(HT29) and CD151 gene knockout colon cancer cells(CD151--HT29) in CD151,LRG-1 and LGR-5 protein expression;the expression of LRG-1 HT29,CD151--HT29 cells and LGR-5 gene were detected by real-time quantitative PCR technique;the invasion ability of HT29,CD151--HT29 cells was inspected.Results The results showed that there was CD151,LRG-1 and LGR-5 protein expression in HT29 cells,but no CD151 protein expression in CD151--HT29 cells,and significantly decrease could be observed in the expression of LRG-1 and LGR-5 protein;real-time quantitative PCR results showe that,compared with HT29 cells,the expression of LRG-1 and LGR-5 gene in CD151--HT29 cells decreased significantly(P < 0.05).Cell invasion assay showed that HT29 cell invasion ability was stronger than that in CD151--HT29 cells(P < 0.05).Conclusion The expression of CD151 gene deletion can inhibit Wnt signaling initiation protein LRG-1 and LGR-5,and decrease the cancer cell invasion,which indicates that the CD151 gene has some role in the regulation of Wnt signaling pathway,to provide some scientific guidance for the colorectal cancer combined with targeted therapy.更多还原