Objective: To establish a method of liquid chromatography-atomic fluorescence spectrometry(LC-AFS), and carry out uncertainty analysis of detecting methyl mercury in fish tissue. Methods: LC-AFS method was adopted to determine the content of methyl mercury in fish tissue. And the theory of assessment and expression about uncertainty measurement based on JJF1059.1-2012 of State Administration for Market Regulation was applied to analyze the source of factors that affect its uncertainty. Through evaluated var...