【摘要】 目的通过对临床分离的幽门螺杆菌(Helicobacter pylori,Hp)进行培养和药敏试验,结合基因检测的方法,探讨Hp的毒力基因Vac A多态性与其耐药性之间的关联。方法采集120例消化道疾病患者的胃黏膜组织,进行Hp分离培养和药敏试验,同时对Hp阳性且有抗生素耐药结果的菌株进行核酸提取,PCR检测Hp毒力基因VacA s区和m区的基因型。采用χ~2检验对Hp毒力基因的类型与其抗生素耐药性间相关性进行评价。结果 56例患者经Hp培养阳性,阳性率46.7%。其中克拉霉素耐药率达到了28.6%。s1/m2型的菌株中,克拉霉素耐药率为43.5%,而s1/m1型的菌株中,克拉霉素耐药率为18.2%,两者之间的差异有统计学意义(P<0.05)。结论 s1/m2型菌株比s1/m1型菌株更容易产生克拉霉素耐药性,Vac A基因可能与Hp对克拉霉素产生耐药性相关。更多还原

【Abstract】 Objective To investigate the relationship between the virulence gene polymorphism of Helicobacter pylori( Hp) and its drug resistance by culturing Hp isolated from clinical and drug susceptibility test and combining with gene detection methods. Methods Gastric mucosa specimens were collected from 120 patients with digestive tract diseases. Hp was cultured and tested for antibiotic susceptibility. The nucleic acid was extracted from Hp positive strains with antibiotic resistance results and the Hp virulence gene VacA s region and m region were detected by PCR. The correction between the polymorphism of virulence genes and its drug resistance was evaluated by the χ~2 test. Results The positive rate was 46. 7% in 56 patients with Hp culture. Clarithromycin resistance rate reached 28. 6%. Clarithromycin resistance rate was 43. 5% in s1/m2 type strains,while in s1/m1 type strains,clarithromycin resistance rate was 18. 2%,the difference was statistically significant( P < 0. 05). Conclusion s1/m2 type strains are more prone to clarithromycin resistance than s1/m1 strains,and the gene polymorphism may be related to Hp resistance to clarithromycin.更多还原